Gas-chromatographic analysis of patterns of fatty acids of cholesteryl esters and phosphatidylcholine.

نویسندگان

  • R A Rockerbie
  • R D Dobson
  • J Frohlich
چکیده

We have developed a quick, reliable method for compositional analysis of the C14 to C20 fatty acids of cholesteryl esters, based on the use of a double extraction-solvent system and whisker-walled capillary columns with a new polar phase. Fatty acids of phosphatidylcholine, after separation by thin-layer chromatography, were determined on the same column. Our normal values are in good agreement with those published previously. We found marked changes in the fatty acid composition of both cholesteryl esters and phosphatidylcholine in cord blood serum, in various disease states, and in post-mortem specimens. The previously reported correlation between oleic (18:1) and linoleic (18:2) acids in serum cholesteryl esters was confirmed for normal adults, but no such correlation exists in cord blood specimens. We also found a significant correlation between the relative amounts of fatty acids in cholesteryl esters and phosphatidylcholine for all unsaturated fatty acids and for palmitate in all groups of subjects.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Composition of the neutral lipids of bovine meilbomian secretions.

Lipids secreted form the bovine meibomian glands were assigned to following classes: cholesteryl esters (A) (fatty acyl chain lengths from 15 to 27 carbon atoms), 41%; wax esters, 29%; triacylglycerols, 10%; and cholesteryl ester (B) (fatty acyl chain lengths from 14 to 18 carbons), 15%. The remaining 5% consisted of cholesterol, fatty acids, and highly polar material. Analysis of the lipids sh...

متن کامل

Studies on Chemical Composition and Nutritive Evaluation of Wild Edible Mushrooms

Agaricus bisporus and Agaricus bitorquis are among most favorite mushrooms of the world. Mineral contents, proximate composition and fatty acid profile of these mushrooms were determined for evaluation of their dietary value. Na, K and Li contents were high, while Cr, Cu, Pb, Co, Zn, Mn and Ni concentrations were low in both Agaricus bisporus and Agaricus bitorquis. Proximate analysis showe...

متن کامل

Polyunsaturated fatty acid concentrations in young men and women consuming their habitual diets.

Young women of reproductive age appear to have a greater capacity than men to convert the essential fatty acid alpha-linolenic acid to DHA. The purpose of this study was to test the hypothesis that gender-related differences in n-3 PUFA metabolism are reflected in the concentrations of n-3 PUFA in plasma lipids. The subjects were healthy men (n 13) and women (n 23) aged 18-35 years consuming th...

متن کامل

Development of gas chromatography analysis of fatty acids in marine organisms.

The gas chromatographic analysis of fatty acids has attracted considerable interest. In this analysis, the common derivatives of fatty acids, such as fatty acid methyl esters, can be detected using a flame ionization detector and the mass spectra can indicate the true structure of fatty acids. This paper reviews gas chromatographic methods for obtaining fatty acids from marine organisms. The st...

متن کامل

Analysis of fatty acid composition of Withania coagulans fruits by gas chromatography/mass spectrometry

Background and objectives: Withania coagulans Dunal (Solanaceae) fruits are recommended to treat asthma, dyspepsia, biliousness, flatulent colic, liver complaints, intestinal infections, strangury, wounds and as diuretic, emetic and sedative agent in Indian traditional system of medicine. The objective of this study was to describe the systematic fatty acid composition...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Clinical chemistry

دوره 25 8  شماره 

صفحات  -

تاریخ انتشار 1979